-
Product Name
Protein A Rabbit pAb (Biotin)
-
Gene ID
3919448
-
SwissProt ID
P02976
-
Gene Name
spa
-
Alternative Names
Immunoglobulin G-binding protein A,IgG-binding protein A,Staphylococcal protein A (SpA),spa
-
Background
Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus (SPA). SPA binds proteins from many of mammalian species, most notably IgGs, and helps inhibit phagocytic engulfment and acts as an immunological disguise via this type of interaction, thus the bacteria will disrupts opsonization and phagocytosis. SPA is known to bind with Fc region of immunoglobulins preferentially through interaction with the VH3 variable region of the heavy chain. SPA has been shown to bind with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b, whereas bind with moderate affinity to human IgM, IgA and IgE as well as mouse IgG3 and IgG1. SPA is often produced in E. coli and is practically coupled to other molecules such as enzymes, biotin, radioactive iodine for use in immunology and other biological research. SPA is also immobilized onto solid supports such as agarose beads for total IgG purifying or interest protein or protein complex identifying in immunoprecipitation studies.
-
Research Field
Tags & Cell Markers
-
Product Categories
Primary antibody
-
Host
Rabbit
-
Reactivity
S.aureus
-
Application
WB,IHC-F,IHC-P,ELISA
-
Dilution Ratio
WB: 1/500-1/1000 IHC: 1/100-1/200 ELISA: 1/5000-1/10000
-
Molecular Weight
40-60 kDa
-
Clonality
Polyclonal Antibody
-
Clonality No.
-
-
Isotype
IgG
-
Immunogen
Recombinant protein A
-
Purification
Affinity purified by Protein A
-
Conjugation
Biotin
-
Modification
Unmodified
-
Form
Liquid
-
Buffer System
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
-
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.