-
Product Name
Phospho-AMPK alpha 1 (Ser486) Rabbit pAb
-
Gene ID
5562
-
SwissProt ID
Q13131
-
Gene Name
PRKAA1
-
Alternative Names
5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; au-protein kinase PRKAA1; PRKAA1; AMPK1
-
Background
AMPK is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKα1 and AMPKα2 genes encode 548 amino acid and 552 amino acid proteins, respectively. Human AMPKβ1 encodes a 271 amino acid protein and human AMPKβ2 encodes a 272 amino acid protein. The human AMPKγ1 gene encodes a 331 amino acid protein. Human AMPKγ2 and AMPKγ3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex.
-
Research Field
-
-
Product Categories
Primary antibody
-
Host
Rabbit
-
Reactivity
Human,Mouse,Rat
-
Application
WB, IHC-P
-
Dilution Ratio
WB: 1/500-1/1000 IHC: 1/50-1/200
-
Molecular Weight
-
-
Clonality
Polyclonal Antibody
-
Clonality No.
-
-
Isotype
IgG
-
Immunogen
Synthetic phosphopeptide derived from human AMPKα1 around the phosphorylation site of Serine 486.
-
Purification
Affinity Purified
-
Conjugation
Unconjugated
-
Modification
Phosphorylated
-
Form
Liquid
-
Buffer System
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
-
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.