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Product Name
RICTOR Mouse mAb
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Gene ID
253260
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SwissProt ID
Q6R327
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Gene Name
RICTOR
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Alternative Names
PIA; mAVO3; KIAA1999; MGC39830; DKFZp686B11164; RICTOR
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Background
Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals . mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, Sin1 and rictor . The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro . This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes . This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR .
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Research Field
Cell Biology
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Product Categories
Primary antibody
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Host
Mouse
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Reactivity
Human,Monkey,Mouse
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Application
WB,IHC-P,ICC/IF,FC
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Dilution Ratio
WB: 1/500-1/1000 IHC: 1/100-1/200 IF: 1/50-1/200 FC: 1/50-1/100
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Molecular Weight
Calculated MW: 192 kDa; Observed MW: 192 kDa
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Clonality
Monoclonal Antibody
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Clonality No.
7B3
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Isotype
IgG1
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Immunogen
Purified recombinant fragment of human RICTOR expressed in E. Coli.
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Purification
Ascitic Fluid
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
Liquid
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Buffer System
Ascitic fluid containing 0.03% sodium azide.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.