-
Product Name
Topoisomerase II alpha Mouse mAb
-
Gene ID
7153
-
SwissProt ID
P11388
-
Gene Name
TOP2A
-
Alternative Names
TOP2; TP2A
-
Background
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.
-
Research Field
Epigenetics and Nuclear Signaling
-
Product Categories
Primary antibody
-
Host
Mouse
-
Reactivity
Human,Rat
-
Application
WB,ICC/IF,FC,ELISA
-
Dilution Ratio
WB: 1/500-1/1000 IF: 1/50-1/200 FC: 1/50-1/100 ELISA: 1/10000
-
Molecular Weight
Calculated MW: 174kDa;Observed MW: 174kDa
-
Clonality
Monoclonal Antibody
-
Clonality No.
1Q3-M5-T3
-
Isotype
Mouse IgG2b
-
Immunogen
Purified recombinant fragment of human TOP2A expressed in E. Coli.
-
Purification
Affinity Purified
-
Conjugation
Unconjugated
-
Modification
Unmodified
-
Form
Liquid
-
Buffer System
Purified antibody in PBS with 0.05% sodium azide
-
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.