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Product Name
LC3A/B (3E9) Mouse mAb
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Gene ID
84557/81631
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SwissProt ID
Q9H492/Q9GZQ8
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Gene Name
MAP1LC3A/MAP1LC3B
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Alternative Names
LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3; MAP1LC3A; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3; MAP1LC3B
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Background
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
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Research Field
Signal Transduction
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Product Categories
Primary antibody
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Host
Mouse
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Reactivity
Human,Rat
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Application
WB
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Dilution Ratio
WB: 1/500-1/1000
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Molecular Weight
Calculated MW: 14 kDa; Observed MW: 14,16 kDa
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Clonality
Monoclonal Antibody
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Clonality No.
3E9-E5-C9
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Isotype
IgG2b
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Immunogen
Synthetic peptide corresponding to human LC3B protein
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
Liquid
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Buffer System
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.