-
Product Name
SIRT1 Mouse mAb
-
Gene ID
23411
-
SwissProt ID
Q96EB6
-
Gene Name
SIRT1
-
Alternative Names
SIR2L1; SIRT1
-
Background
The Sir2 protein in yeast is known to function in transcriptional silencing processes through the deacetylation of histones H3 and H4. The more recently described human homologue of Sir2, known as SIRT1, has been found to associate with the tumor suppressor protein p53.SIRT1 binds and deacetylates p53 with specificity for its C-terminal Lys382 residue in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras. The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress.SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues, with high expression in heart, brain and skeletal muscle and low expression in lung and placenta. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.
-
Research Field
Cell Biology
-
Product Categories
Primary antibody
-
Host
Mouse
-
Reactivity
Human
-
Application
WB,IHC-P,ICC/IF,FC
-
Dilution Ratio
WB: 1/500-1/1000 IHC: 1/100-1/200 IF: 1/50-1/200 FC: 1/50-1/100
-
Molecular Weight
Calculated MW: 82 kDa;Observed MW: 110 kDa
-
Clonality
Monoclonal Antibody
-
Clonality No.
4E2-H9-M8
-
Isotype
Mouse IgG1
-
Immunogen
Purified recombinant fragment of human SIRT1 expressed in E. Coli.
-
Purification
Affinity Purified
-
Conjugation
Unconjugated
-
Modification
Unmodified
-
Form
Liquid
-
Buffer System
Ascitic fluid containing 0.03% sodium azide.
-
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.