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Product Name
RPA32 Mouse mAb
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Gene ID
6118
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SwissProt ID
P15927
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Gene Name
RFA2
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Alternative Names
RP-A p34, RPA2, REPA2, RPA32, RPA34
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Background
This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways.
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Research Field
Epigenetics and Nuclear Signaling
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Product Categories
Primary antibody
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Host
Mouse
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Reactivity
Human
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Application
WB,IHC-P,FC
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Dilution Ratio
WB: 1/500-1/1000 IHC: 1/100-1/200 FC: 1/50-1/100
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Molecular Weight
Calculated MW: 29 kDa;Observed MW: 32 kDa
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Clonality
Monoclonal Antibody
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Clonality No.
9B2-O4-Q6
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Isotype
Mouse IgG1,k
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Immunogen
Purified recombinant fragment of human RFA2 expressed in E. Coli.
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
Liquid
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Buffer System
Purified antibody in PBS with 0.05% sodium azide
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.