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Product Name
SAFB1 (2E8) Mouse mAb
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Gene ID
6294
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SwissProt ID
Q15424
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Gene Name
SAFB
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Alternative Names
SAFB; HAP; HET; SAFB1; Scaffold attachment factor B1; SAF-B; SAF-B1; HSP27 estrogen response element-TATA box-binding protein; HSP27 ERE-TATA-binding protein
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Background
This gene encodes a DNA-binding protein which has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. The encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressor and is a candidate for breast tumorigenesis. This gene is arranged head-to-head with a similar gene whose product has the same functions. Multiple transcript variants encoding different isoforms have been found for this gene.
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Research Field
Epigenetics and Nuclear Signaling
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Product Categories
Primary antibody
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Host
Mouse
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Reactivity
Human
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Application
WB,ICC/IF,ChIP
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Dilution Ratio
WB: 1/500-1/1000 IF: 1/50-1/200 ChIP: 1/20
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Molecular Weight
Calculated MW: 103 kDa; Observed MW: 130 kDa
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Clonality
Monoclonal Antibody
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Clonality No.
2E8-E2-G6
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Isotype
IgG1
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Immunogen
Purified recombinant human SAFB1 protein fragments expressed in E.coli.
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
Liquid
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Buffer System
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.