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Product Name
Nucleophosmin (2D9) Mouse mAb
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Gene ID
4869
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SwissProt ID
P06748
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Gene Name
NPM1
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Alternative Names
B23; NPM
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Background
Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486). In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029).
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Research Field
Epigenetics and Nuclear Signaling
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Product Categories
Primary antibody
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Host
Mouse
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Reactivity
Human,Mouse
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Application
WB,ChIP
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Dilution Ratio
WB: 1/500-1/1000 ChIP: 1/20
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Molecular Weight
Calculated MW: 33 kDa; Observed MW: 38 kDa
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Clonality
Monoclonal Antibody
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Clonality No.
2D9-C3-E5
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Isotype
IgG1
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Immunogen
Purified recombinant full length of human NPM1 protein expressed in E.coli.
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
Liquid
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Buffer System
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.