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Product Name
Phospho-Src (Tyr530) Rabbit pAb
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Gene ID
6714
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SwissProt ID
P12931
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Gene Name
SRC
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Alternative Names
SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src
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Background
Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).
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Research Field
Signal Transduction
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Product Categories
Primary antibody
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Host
Rabbit
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Reactivity
Human,Mouse,Rat
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Application
WB,IHC-P,ICC/IF
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Dilution Ratio
WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
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Molecular Weight
Calculated MW: 60 kDa; Observed MW: 60 kDa
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Clonality
Polyclonal Antibody
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Clonality No.
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Isotype
IgG
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Immunogen
Peptide sequence around phosphorylation site of tyrosine 529 (P-Q-Y(p)-Q-P) derived from Human Src.
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Purification
Affinity Chromatography
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Conjugation
Unconjugated
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Modification
Phosphorylated
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Form
Liquid
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Buffer System
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.