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Product Name
BTK Mouse mAb
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Gene ID
695
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SwissProt ID
Q06187
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Gene Name
BTK
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Alternative Names
BTK
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Background
Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases.All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini.BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation.
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Research Field
Signal Transduction
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Product Categories
Primary antibody
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Host
Mouse
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Reactivity
Human,Monkey
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Application
WB,IHC-P,ICC/IF
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Dilution Ratio
WB: 1/500-1/1000 IHC: 1/100-1/200 IF: 1/50-1/200
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Molecular Weight
Calculated MW: 76 kDa;Observed MW: 90-130 kDa
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Clonality
Monoclonal Antibody
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Clonality No.
7Y1-X7-T9
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Isotype
Mouse IgG1
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Immunogen
Purified recombinant fragment of BTK expressed in E. Coli.
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
Liquid
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Buffer System
Ascitic fluid containing 0.03% sodium azide.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.