产品详情
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Product Name
SETD7 Mouse mAb
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Gene ID
80854
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SwissProt ID
Q8WTS6
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Gene Name
SETD7
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Alternative Names
KMT7; SET7; SET9; SET7/9
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Background
Histone methyltransferase that specifically monomethylates Lys-4 of histone H3. H3 Lys-4 methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates Lys-189 of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates Lys-372 of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation.
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Research Field
Epigenetics and Nuclear Signaling
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Product Categories
Primary antibody
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Host
Mouse
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Reactivity
Human,Rat,Monkey
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Application
WB,IHC-P,ELISA
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Dilution Ratio
WB: 1/500-1/1000 IHC: 1/100-1/200 ELISA: 1/10000
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Molecular Weight
Calculated MW: 41 kDa
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Clonality
Monoclonal Antibody
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Clonality No.
5M1-T2-P3
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Isotype
Mouse IgG1
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Immunogen
Purified recombinant fragment of human SETD7 expressed in E. Coli.
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
Liquid
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Buffer System
Purified antibody in PBS with 0.05% sodium azide
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
应用缩写
WB:蛋白质印迹法IHC-P:石蜡切片样本的免疫组织化学法IHC-F:冰冻切片样本的免疫组织化学法ICC/IF:免疫细胞荧光 IP:免疫沉淀法FC:流式细胞术ChIP:染色质免疫沉淀法ELISA:酶联免疫吸附测定
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