产品详情
-
Product Name
ATG13 Mouse mAb
-
Gene ID
9776
-
SwissProt ID
O75143
-
Gene Name
ATG13
-
Alternative Names
KIAA0652
-
Background
ATG13 is a protein kinase required for autophagy. Overexpression of Atg1 suppresses the defects in autophagy observed in Atg13 mutants. Autophagy requires a direct association between Atg1 and Atg13, and is inhibited by TOR-dependent phosphorylation of Atg13 under high-nutrient conditions. Similarly, mammalian Atg13 forms a complex with the Atg1 homologues ULK1/2, along with FIP200, which localizes to autophagic isolation membranes and regulates autophagosome biogenesis. mTOR phosphorylates both Atg13 and ULK1, suppressing ULK1 kinase activity and autophagy. ULK1 can directly phosphorylate Atg13 at a yet unidentified site, presumably to promote autophagy. Additional studies suggest that Atg13 and FIP200 can function independently of ULK1 and ULK2 to induce autophagy through an unknown mechanism.
-
Research Field
Tumor biology
-
Product Categories
Primary antibody
-
Host
Mouse
-
Reactivity
Human,Mouse
-
Application
WB,IHC-P
-
Dilution Ratio
WB：1/500-1/1000 IHC-P：1/50-1/100
-
Molecular Weight
Calculated MW: 57 kDa; Observed MW:57 kDa
-
Clonality
Monoclonal Antibody
-
Clonality No.
2X9-F9-I4
-
Isotype
IgG1/Kappa
-
Immunogen
Peptide
-
Purification
Protein A
-
Conjugation
Unconjugated
-
Modification
Unmodified
-
Form
Liquid
-
Buffer System
Liquid in PBS, Glycerol and BSA
-
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
应用缩写
WB:蛋白质印迹法IHC-P:石蜡切片样本的免疫组织化学法IHC-F:冰冻切片样本的免疫组织化学法ICC/IF:免疫细胞荧光 IP:免疫沉淀法FC:流式细胞术ChIP:染色质免疫沉淀法ELISA:酶联免疫吸附测定
正能生物产品售后保障范围仅限官网产品详情页中“应用”一栏写明的实验应用，请参照上方的缩写含义，仔细对照后选择合适的产品。