产品详情
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Product Name
ATM Mouse mAb
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Gene ID
472
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SwissProt ID
Q13315
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Gene Name
ATM
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Alternative Names
–
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Background
Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control.
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Research Field
Tumor biology
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Product Categories
Primary antibody
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Host
Mouse
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Reactivity
Human
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Application
WB
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Dilution Ratio
WB：1/500-1/1000
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Molecular Weight
Calculated MW: 350 kDa; Observed MW:350 kDa
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Clonality
Monoclonal Antibody
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Clonality No.
8W5-P3-E3
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Isotype
IgG1/Kappa
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Immunogen
Peptide
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Purification
Protein G
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
Liquid
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Buffer System
Liquid in PBS, Glycerol and BSA
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
应用缩写
WB:蛋白质印迹法IHC-P:石蜡切片样本的免疫组织化学法IHC-F:冰冻切片样本的免疫组织化学法ICC/IF:免疫细胞荧光 IP:免疫沉淀法FC:流式细胞术ChIP:染色质免疫沉淀法ELISA:酶联免疫吸附测定
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