-
Product Name
Phospho-BRAF (Thr401) Rabbit mAb
-
Gene ID
673
-
SwissProt ID
P15056
-
Gene Name
BRAF
-
Alternative Names
BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1
-
Background
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations.
-
Research Field
Cell Biology
-
Product Categories
Primary antibody
-
Host
Rabbit
-
Reactivity
Human,Mouse,Rat
-
Application
WB,IP
-
Dilution Ratio
WB: 1/500-1/1000 IP: 1/20
-
Molecular Weight
Calculated MW: 84 kDa; Observed MW: 84 kDa
-
Clonality
Monoclonal Antibody
-
Clonality No.
R05-9B6
-
Isotype
IgG
-
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding Thr401 of human B Raf
-
Purification
Affinity Purified
-
Conjugation
Unconjugated
-
Modification
Phosphorylated
-
Form
Liquid
-
Buffer System
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
-
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.