-
Product Name
ATG4A Rabbit mAb
-
Gene ID
115201
-
SwissProt ID
Q8WYN0
-
Gene Name
ATG4A
-
Alternative Names
ATG4A; APG4A; AUTL2; Cysteine protease ATG4A; AUT-like 2 cysteine endopeptidase; Autophagin-2; Autophagy-related cysteine endopeptidase 2; Autophagy-related protein 4 homolog A; hAPG4A
-
Background
The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homologue for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homologue is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homologue.
-
Research Field
Cell Biology
-
Product Categories
Primary antibody
-
Host
Rabbit
-
Reactivity
Human
-
Application
WB,IHC-P,IP
-
Dilution Ratio
WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20
-
Molecular Weight
Calculated MW: 45 kDa; Observed MW: 45 kDa
-
Clonality
Monoclonal Antibody
-
Clonality No.
R05-3H3
-
Isotype
IgG
-
Immunogen
A synthetic peptide of human ATG4A
-
Purification
Affinity Purified
-
Conjugation
Unconjugated
-
Modification
Unmodified
-
Form
Liquid
-
Buffer System
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
-
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.