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Product Name
APPBP1 Rabbit mAb
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Gene ID
8883
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SwissProt ID
Q13564
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Gene Name
NAE1
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Alternative Names
NEDD8 activating enzyme E1 subunit 1; HPP1; ula-1; APPBP1; A-116A10.1
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Background
Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.
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Research Field
Cell Biology
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Product Categories
Primary antibody
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Host
Rabbit
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Reactivity
Human
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Application
WB,IP
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Dilution Ratio
WB: 1/500-1/1000 IP: 1/20
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Molecular Weight
Calculated MW: 60 kDa; Observed MW: 60 kDa
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Clonality
Monoclonal Antibody
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Clonality No.
R03-1A7
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Isotype
IgG
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Immunogen
A synthetic peptide of human APPBP1
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
Liquid
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Buffer System
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.