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Product Name
Phospho-Rb (Ser780) Rabbit mAb
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Gene ID
5925
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SwissProt ID
P06400
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Gene Name
RB1
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Alternative Names
RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110
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Background
Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.
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Research Field
Cell Biology
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Product Categories
Primary antibody
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Host
Rabbit
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Reactivity
Human,Rat
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Application
WB,IHC-P
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Dilution Ratio
WB: 1/500-1/1000 IHC: 1/50-1/100
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Molecular Weight
Calculated MW: 106 kDa; Observed MW: 110 kDa
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Clonality
Monoclonal Antibody
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Clonality No.
R04-5B7
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Isotype
IgG
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Immunogen
A synthetic phosphopeptide corresponding to residues surrounding Ser780 of human Rb
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Phosphorylated
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Form
Liquid
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Buffer System
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.