-
Product Name
Tight Junction Protein ZO 3 Rabbit mAb
-
Gene ID
27134
-
SwissProt ID
O95049
-
Gene Name
TJP3
-
Alternative Names
ZO3; ZO-3
-
Background
TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed:16129888). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Binds and recruits PATJ to tight junctions where it connects and stabilizes apical and lateral components of tight junctions (PubMed:16129888). Promotes cell-cycle progression through the sequestration of cyclin D1 (CCND1) at tight junctions during mitosis which prevents CCND1 degradation during M-phase and enables S-phase transition (PubMed:21411630). With TJP1 and TJP2, participates to the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus . Contrary to TJP2, TJP3 is dispensable for individual viability, embryonic development, epithelial differentiation, and the establishment of TJs, at least in the laboratory environment .
-
Research Field
Signal Transduction
-
Product Categories
Primary antibody
-
Host
Rabbit
-
Reactivity
Human,Rat
-
Application
WB
-
Dilution Ratio
WB: 1/500-1/1000
-
Molecular Weight
Calculated MW: 101 kDa; Observed MW: 140 kDa
-
Clonality
Monoclonal Antibody
-
Clonality No.
R08-4E8
-
Isotype
IgG
-
Immunogen
A synthetic peptide of human Zonula occludens protein 3
-
Purification
Affinity Purified
-
Conjugation
Unconjugated
-
Modification
Unmodified
-
Form
Liquid
-
Buffer System
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
-
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.