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Product Name
Securin Rabbit mAb
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Gene ID
9232
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SwissProt ID
O95997
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Gene Name
PTTG1
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Alternative Names
PTTG1; EAP1; PTTG; TUTR1; Securin; Esp1-associated protein; Pituitary tumor-transforming gene 1 protein; Tumor-transforming protein 1; hPTTG
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Background
Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.
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Research Field
Cell Biology
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Product Categories
Primary antibody
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Host
Rabbit
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Reactivity
Human,Mouse
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Application
WB,IHC-P,IP
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Dilution Ratio
WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20
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Molecular Weight
Calculated MW: 22 kDa; Observed MW: 25 kDa
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Clonality
Monoclonal Antibody
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Clonality No.
R09-6J7
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Isotype
IgG
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Immunogen
A synthetic peptide of human Securin
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
Liquid
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Buffer System
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.