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Product Name
Firefly Luciferase Rabbit mAb
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Gene ID
116160065
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SwissProt ID
P08659
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Gene Name
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Alternative Names
Luciferase; Firefly; Luciferin 4 monooxygenase
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Background
Produces green light with a wavelength of 562 nm. Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
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Research Field
Tags & Cell Markers
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Product Categories
Primary antibody
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Host
Rabbit
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Reactivity
Firefly
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Application
WB,ICC/IF
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Dilution Ratio
WB: 1/500-1/1000 IF: 1/50-1/200
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Molecular Weight
Calculated MW: 61 kDa
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Clonality
Monoclonal Antibody
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Clonality No.
R09-8F1
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Isotype
IgG
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Immunogen
A synthesized peptide derived from Firefly Luciferase
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Purification
Affinity Chromatography
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
Liquid
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Buffer System
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.