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Product Name
Phospho-GSK3 (Tyr216/Tyr279) Rabbit mAb
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Gene ID
2931/2932
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SwissProt ID
P49840/P49841
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Gene Name
GSK3A/GSK3B
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Alternative Names
Serine/threonine-protein kinase GSK3A; Serine/threonine-protein kinase GSK3B
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Background
Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1. Requires primed phosphorylation of the majority of its substrates. Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle. May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease. May be involved in the regulation of replication in pancreatic beta-cells. Is necessary for the establishment of neuronal polarity and axon outgrowth. Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation.
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Research Field
Neuroscience
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Product Categories
Primary antibody
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Host
Rabbit
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Reactivity
Mouse,Rat
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Application
WB,IP
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Dilution Ratio
WB: 1/500-1/1000 IP: 1/20
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Molecular Weight
Calculated MW: 51 kDa; Observed MW: 47-51 kDa
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Clonality
Monoclonal Antibody
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Clonality No.
R01-5G6
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Isotype
IgG
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Immunogen
A synthetic phosphopeptide corresponding to residues surrounding Tyr216 of human GSK3 alpha
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Phosphorylated
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Form
Liquid
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Buffer System
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.