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Product Name
EEA1 Rabbit mAb
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Gene ID
8411
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SwissProt ID
Q15075
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Gene Name
EEA1
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Alternative Names
Early endosome antigen 1; EEA1; MST105; ZFYVE2;
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Background
EEA1 is an early endosomal marker and a Rab5 effector protein essential for early endosomal membrane fusion and trafficking (1-2). The carboxy terminus of EEA1 contains a FYVE domain which binds to phosphatidylinositol-3-phosphate (PtdIns(3)P), targeting EEA1 to early endosomes (3). The stable association of EEA1 with the endosomal membrane is regulated by PI3 kinase, Rab5 and calcium/calmodulin (4-6). Once on the membrane, EEA1 interacts with Rab5, NSF and syntaxin 13 to promote early endosomal membrane docking and fusion (7).
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Research Field
Neuroscience
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Product Categories
Primary antibody
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Host
Rabbit
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Reactivity
Human,Mouse,Rat
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Application
WB,ICC/IF
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Dilution Ratio
WB: 1/500-1/1000 IF: 1/50-1/200
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Molecular Weight
Calculated MW: 162 kDa;Observed MW:170kDa
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Clonality
Monoclonal Antibody
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Clonality No.
R25-6Z-9
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Isotype
IgG
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Immunogen
A synthesized peptide derived from human EEA1
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Purification
Affinity Chromatography
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
Liquid
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Buffer System
Liquid in PBS , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.