-
Product Name
Phospho-RIP (S166) Rabbit mAb
-
Gene ID
19766
-
SwissProt ID
Q60855
-
Gene Name
Ripk1
-
Alternative Names
Cell death protein RIP,Receptor-interacting protein 1
-
Background
Receptor-interacting serine/threonine-protein kinase 1 is a serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. RIPK1 is phosphorylated at several sites within the kinase domain that are sensitive to Nec-1, including Ser14, Ser15, Ser161, and Ser166.
-
Research Field
Cell Biology
-
Product Categories
Primary antibody
-
Host
Rabbit
-
Reactivity
Mouse
-
Application
WB,IHC-F,IHC-P,ICC/IF,IP
-
Dilution Ratio
WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/50-1/500
-
Molecular Weight
Calculated MW: 75 kDa,Observed MW:75 kDa
-
Clonality
Monoclonal Antibody
-
Clonality No.
R03-3P4
-
Isotype
IgG
-
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding serine 166 of Mouse RIPK1
-
Purification
ProA affinity purified IgG
-
Conjugation
Unconjugated
-
Modification
Phosphorylated
-
Form
Liquid
-
Buffer System
Liquid in PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA
-
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.