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Product Name
Loading Control Rabbit pAb Kit
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Gene ID
60/203068/2597
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SwissProt ID
P60709/P07437/P04406
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Gene Name
ACTB/TUBB/GAPDH
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Alternative Names
ACTIN; ACTB; BETA-ACTIN; beta actin; actin beta; Beta 4 tubulin; TBB5; TUBB2; TUBB2A; tubulin beta 2A; beta tubulin; tubulin beta chain; G3PD; GAPD; GAPDH; HEL-S-162eP
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Background
This kit is consist of three different but reliable antibodies which are specific to the most popular three loading control targets: GAPDH, β-Actin and β-Tubulin, also with three individual package.
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells(Swiss-Prot Acc.P60709).
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain (Swiss-Prot Acc.P07437).
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue, GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis, gene expression, and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer diseases (Swiss-Prot Acc.P04406).
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Research Field
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Product Categories
Loading control antibody
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Host
Rabbit
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Reactivity
Human,Mouse,Rat
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Application
WB,IHC-P,ICC/IF
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Dilution Ratio
WB: 1/5000-1/10000 IHC: 1/50-1/200 IF: 1/100-1/200
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Molecular Weight
Beta actin: 42 kDa; Beta Tubulin: 55 kDa; GAPDH: 36 kDa
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Clonality
Polyclonal Antibody
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Clonality No.
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Isotype
IgG
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Immunogen
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Purification
Affinity Chromatography
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
liquid
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Buffer System
Beta actin: Rabbit IgG in phosphate buffered saline,pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Beta Tubulin: Rabbit IgG in phosphate buffered saline,pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
GAPDH: Rabbit IgG in phosphate buffered saline,pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.