产品详情
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Product Name
GAPDH Rabbit pAb
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Gene ID
2597
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SwissProt ID
P04406
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Gene Name
GAPDH
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Alternative Names
G3PD; GAPD; HEL-S-162eP
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Background
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.
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Research Field
Neuroscience
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Product Categories
Loading Control antibody
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Host
Rabbit
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Reactivity
Human,Mouse,Rat
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Application
WB
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Dilution Ratio
WB: 1/5000-1/10000
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Molecular Weight
Calculated MW: 36 kDa; Observed MW: 36 kDa
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Clonality
Polyclonal Antibody
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Clonality No.
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Isotype
IgG
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Immunogen
Purified recombinant Human GAPDH protein fragments expressed in E.coli.
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
Liquid
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Buffer System
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
应用缩写
WB:蛋白质印迹法IHC-P:石蜡切片样本的免疫组织化学法IHC-F:冰冻切片样本的免疫组织化学法ICC/IF:免疫细胞荧光 IP:免疫沉淀法FC:流式细胞术ChIP:染色质免疫沉淀法ELISA:酶联免疫吸附测定
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样本种属:
Human
杂 志:
Journal of Cancer
应用:
WB
IF:
3.9
样本种属:
Human
杂 志:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
应用:
WB
IF:
3.322
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