货号： R24404
别名： G3PD; GAPD; HEL-S-162eP
应用： WB,IHC-F,IHC-P,ICC/IF
反应种属： Human,Mouse,Rat,Hamster
目录价格：
RMB(含税报价)
数量： - + （库存件）
查看代理商 预计货期：现货 说明书PDF
产品详情
  • Product Name
    GAPDH Rabbit mAb
  • Gene ID
    2597
  • SwissProt ID
    P04406
  • Gene Name
    GAPDH
  • Alternative Names
    G3PD; GAPD; HEL-S-162eP
  • Background
    Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.
  • Research Field
    Neuroscience
  • Product Categories
    Loading Control antibody
  • Host
    Rabbit
  • Reactivity
    Human,Mouse,Rat,Hamster
  • Application
    WB,IHC-F,IHC-P,ICC/IF
  • Cited
    WB (Chicken)
  • Dilution Ratio
    WB: 1/5000-1/10000 IHC: 1/50-1/200 IF: 1/100-1/200
  • Molecular Weight
    Calculated MW: 36 kDa; Observed MW: 36 kDa
  • Clonality
    Monoclonal Antibody
  • Clonality No.
    R08-4C3
  • Isotype
    IgG
  • Immunogen
    Recombinant protein of human GAPDH
  • Purification
    Affinity Purified
  • Conjugation
    Unconjugated
  • Modification
    Unmodified
  • Form
    Liquid
  • Buffer System
    50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
  • Storage
    Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
验证详情

应用缩写

WB:蛋白质印迹法IHC-P:石蜡切片样本的免疫组织化学法IHC-F:冰冻切片样本的免疫组织化学法ICC/IF:免疫细胞荧光 IP:免疫沉淀法FC:流式细胞术ChIP:染色质免疫沉淀法ELISA:酶联免疫吸附测定 正能生物产品售后保障范围仅限官网产品详情页中“应用”一栏写明的实验应用，请参照上方的缩写含义，仔细对照后选择合适的产品。
引用文献 更多完整详情
标 题： The natural product salicin alleviates osteoarthritis progression by binding to IRE1α and inhibiting endoplasmic reticulum stress through the IRE1α-IκBα-p65 signaling pathway
样本种属： Rat
杂 志： EXPERIMENTAL AND MOLECULAR MEDICINE
应用： WB
IF： 12.153
文献链接
标 题： Engineered Exosome-Functionalized Extracellular Matrix-Mimicking Hydrogel for Promoting Bone Repair in Glucocorticoid-Induced Osteonecrosis of the Femoral Head
样本种属： Mouse
杂 志： ACS Applied Materials & Interfaces
应用： WB
IF： 9.5
文献链接
标 题： Cuproptosis status affects treatment options about immunotherapy and targeted therapy for patients with kidney renal clear cell carcinoma.
样本种属： Human
杂 志： Frontiers in Immunology
应用： WB
IF： 8.786
文献链接
标 题： Stem cells from human exfoliated deciduous teeth as an alternative cell source in bio-root regeneration
样本种属： Human
杂 志： Theranostics
应用： WB
IF： 8.063
文献链接
标 题： Hypoxia-Induced HIF-1&alpha|| Expression Promotes Neurogenic Bladder Fibrosis via EMT and Pyroptosis
样本种属： Rat
杂 志： Cells
应用： IHC
IF： 7.666
文献链接
标 题： SETD3 is regulated by a couple of microRNAs and plays opposing roles in proliferation and metastasis of hepatocellular carcinoma
样本种属： Mouse,Human
杂 志： CLINICAL SCIENCE
应用： WB
IF： 6.124
文献链接
标 题： Polydopamine-Based Resveratrol-Hyaluronidase Nanomedicine Inhibited Pancreatic Cancer Cell Invasive Phenotype in Hyaluronic Acid Enrichment Tumor Sphere Model
样本种属： Human
杂 志： ACS Pharmacology & Translational Science
应用： WB
IF： 6
文献链接
标 题： TRAF-STOP alleviates osteoclastogenesis in periodontitis
样本种属： ——
杂 志： Frontiers in Pharmacology
应用： WB
IF： 5.988
文献链接
标 题： Hesperetin Exhibits Anti-Inflammatory Effects on Chondrocytes via the AMPK Pathway to Attenuate Anterior Cruciate Ligament Transection-Induced Osteoarthritis
样本种属： Rat
杂 志： Frontiers in Pharmacology
应用： WB
IF： 5.811
文献链接
标 题： Inhibition of interleukin-6 trans-signaling improves survival and prevents cognitive impairment in a mouse model of sepsis
样本种属： Mouse
杂 志： INTERNATIONAL IMMUNOPHARMACOLOGY
应用： WB
IF： 5.714
文献链接
标 题： Activation of estrogen receptor α inhibits TLR4 signaling in macrophages and alleviates the instability of atherosclerotic plaques in the postmenopausal stage
样本种属： Mouse,Human
杂 志： INTERNATIONAL IMMUNOPHARMACOLOGY
应用： IHC,IF,WB
IF： 5.714
文献链接
标 题： Irisin promotes proliferation but inhibits differentiation in osteoclast precursor cells
样本种属： Mouse
杂 志： FASEB JOURNAL
应用： WB
IF： 5.595
文献链接
标 题： Presegetane diterpenoids from Euphorbia sieboldiana as a new type of anti-liver fibrosis agents that inhibit TGF-β/Smad signaling pathway
样本种属： Human
杂 志： BIOORGANIC CHEMISTRY
应用： WB
IF： 5.275
文献链接
标 题： Wuzi-Yanzong-Wan prevents oligoasthenospermia due to TAp73 suppression by affecting cellular junction remodeling in testicular tissue in mice
样本种属： Mouse
杂 志： JOURNAL OF ETHNOPHARMACOLOGY
应用： WB
IF： 5.2
文献链接
标 题： Transcription factor Sp1 is upregulated by PKCι to drive the expression of YAP1 during pancreatic carcinogenesis
样本种属： Human
杂 志： CARCINOGENESIS
应用： WB
IF： 4.944
文献链接
标 题： Paeoniflorin-loaded pH-sensitive liposomes alleviate synovial inflammation by altering macrophage polarity via STAT signaling
样本种属： Rat
杂 志： INTERNATIONAL IMMUNOPHARMACOLOGY
应用： WB
IF： 4.932
文献链接
标 题： Inhibitory effect of the TSG-6 on the BMP-4/Smad signaling pathway and odonto/osteogenic differentiation of dental pulp stem cells
样本种属： Human
杂 志： BIOMEDICINE & PHARMACOTHERAPY
应用： WB
IF： 4.545
文献链接
标 题： Ursolic acid protects chondrocytes, exhibits anti-inflammatory properties via regulation of the NF-κB/NLRP3 inflammasome pathway and ameliorates osteoarthritis
样本种属： Rat
杂 志： BIOMEDICINE & PHARMACOTHERAPY
应用： WB
IF： 4.545
文献链接
标 题： Effect of F11R Gene Knockdown on Malignant Biological Behaviors of Pancreatic Cancer Cells
样本种属： Human
杂 志： Journal of Oncology
应用： WB
IF： 4.501
文献链接
标 题： Desmethoxycurcumin aids IFNα’s anti-HBV activity by antagonising CRYAB reduction and stabilising IFNAR1 protein
样本种属： Human
杂 志： JOURNAL OF DRUG TARGETING
应用： WB
IF： 4.5
文献链接
标 题： Safflower yellow alleviates osteoarthritis and prevents inflammation by inhibiting PGE2 release and regulating NF-κB/SIRT1/AMPK signaling pathways
样本种属： Rat
杂 志： PHYTOMEDICINE
应用： ICC
IF： 4.268
文献链接
标 题： Construction and validation of a novel ferroptosis-related prognostic signature for lung adenocarcinoma
样本种属： Human
杂 志： Translational Lung Cancer Research
应用： WB
IF： 4
文献链接
标 题： Optimal vacuum erectile device therapy regimen for penile rehabilitation in a bilateral cavernous nerve crush rat model
样本种属： Rat
杂 志： Andrology
应用： WB,IHC
IF： 3.842
文献链接
标 题： PDGF-AA promotes gap junction intercellular communication in chondrocytes via the PI3K/Akt pathway
样本种属： Mouse
杂 志： CONNECTIVE TISSUE RESEARCH
应用： WB
IF： 3.417
文献链接
标 题： Preparation of a novel monoclonal antibody against active components of PHA-L from Phaseolus vulgaris and its functional characteristics
样本种属： Human
杂 志： BMC BIOTECHNOLOGY
应用： WB
IF： 3.33
文献链接
标 题： MiR-148a-3p Regulates Skeletal Muscle Satellite Cell Differentiation and Apoptosis via the PI3K/AKT Signaling Pathway by Targeting Meox2
样本种属： Chick
杂 志： Frontiers in Genetics
应用： WB
IF： 3.26
文献链接
标 题： Atg3 Overexpression Enhances Bortezomib-Induced Cell Death in SKM-1 Cell
样本种属： Human
杂 志： PLoS One
应用： WB
IF： 3.057
文献链接
标 题： Sacral Nerve Stimulation Alleviates Intestinal Inflammation Through Regulating the Autophagy of Macrophages and Activating the Inflammasome Mediated by a Cholinergic Antiinflammatory Pathway in Colitis Rats
样本种属： Rat
杂 志： NEUROMODULATION
应用： WB
IF： 3.025
文献链接
标 题： Carvedilol induces pyroptosis through NLRP3-caspase1-ASC inflammasome by nuclear migration of NF-κB in prostate cancer models
样本种属：
杂 志： MOLECULAR BIOLOGY REPORTS
应用：
IF： 2.8
文献链接
标 题： Bioinformatics analysis of KLF2 as a potential prognostic factor in ccRCC and association with epithelial‑mesenchymal transition
样本种属： Human
杂 志： Experimental and Therapeutic Medicine
应用： WB
IF： 2.751
文献链接
标 题： High Expression Level of TRIP6 is Correlated with Poor Prognosis in Colorectal Cancer and Promotes Tumor Cell Proliferation and Migration
样本种属： Human
杂 志： BIOCHEMICAL GENETICS
应用： WB
IF： 2.4
文献链接
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