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货号： R23613

别名： ACTIN; ACTB; BETA-ACTIN; beta actin; actin beta

应用： WB,IHC-F,IHC-P,ICC/IF

反应种属： Human,Mouse,Rat

目录价格：

RMB(含税报价)

数量： - + （库存件）

查看代理商预计货期：现货说明书PDF

产品详情

Product Name

beta Actin Rabbit mAb
Gene ID

60
SwissProt ID

P60709
Gene Name

ACTB
Alternative Names

ACTIN; ACTB; BETA-ACTIN; beta actin; actin beta
Background

beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.
Research Field

Cell Biology
Product Categories

Loading Control antibody
Host

Rabbit
Reactivity

Human,Mouse,Rat
Application

WB,IHC-F,IHC-P,ICC/IF
Cited

WB (Chicken)
Dilution Ratio

WB: 1/5000-1/10000 IHC: 1/50-1/200 IF: 1/100-1/200
Molecular Weight

Calculated MW: 42 kDa; Observed MW: 42 kDa
Clonality

Monoclonal Antibody
Clonality No.

R09-4C9
Isotype

IgG
Immunogen

A synthetic peptide of human beta Actin
Purification

Affinity Purified
Conjugation

Unconjugated
Modification

Unmodified
Form

Liquid
Buffer System

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

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验证详情

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应用缩写

WB:蛋白质印迹法IHC-P:石蜡切片样本的免疫组织化学法IHC-F:冰冻切片样本的免疫组织化学法ICC/IF:免疫细胞荧光 IP:免疫沉淀法FC:流式细胞术ChIP:染色质免疫沉淀法ELISA:酶联免疫吸附测定正能生物产品售后保障范围仅限官网产品详情页中“应用”一栏写明的实验应用，请参照上方的缩写含义，仔细对照后选择合适的产品。

引用文献更多完整详情

标题： Oxidized mitochondrial DNA sensing by STING signaling promotes the antitumor effect of an irradiated immunogenic cancer cell vaccine

样本种属： Mouse

杂志： Cellular & Molecular Immunology

应用： WB

IF： 8.484

文献链接

标题： Lactate Mediates the Bone Anabolic Effect of High-Intensity Interval Training by Inducing Osteoblast Differentiation

样本种属： Mouse

杂志： JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME

应用： WB

IF： 6.558

文献链接

标题： Inhibition of Oncogenic Src Ameliorates Silica-Induced Pulmonary Fibrosis via PI3K/AKT Pathway

样本种属： Mouse

杂志： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

应用： IF,WB

IF： 6.21

文献链接

标题： Hsp90 protected chicken primary myocardial cells from heat-stress injury by inhibiting oxidative stress and calcium overload in mitochondria

样本种属： Chicken

杂志： BIOCHEMICAL PHARMACOLOGY

应用： WB

IF： 6.1

文献链接

标题： Neutrophil extracellular traps promote bronchopulmonary dysplasia-like injury in neonatal mice via the WNT/β-catenin pathway

样本种属： Mouse

杂志： Frontiers in Cellular and Infection Microbiology

应用： WB

IF： 6.073

文献链接

标题： Simulated microgravity attenuates skin wound healing by inhibiting dermal fibroblast migration via F-actin/YAP signaling pathway

样本种属： Human

杂志： JOURNAL OF CELLULAR PHYSIOLOGY

应用： WB

IF： 5.6

文献链接

标题： Phospholipase A2 induces acute kidney injury by complement mediated mitochondrial apoptosis via TNF-α/NF-κB signaling pathway

样本种属： Mouse

杂志： FOOD AND CHEMICAL TOXICOLOGY

应用： WB

IF： 5.6

文献链接

标题： Melanin Nanoparticle-Actuated Redox-State Perturbation and Temporally Photoactivated Thermal Stress for Synergistic Tumor Therapy

样本种属： Human

杂志： ACS Biomaterials Science & Engineering

应用： WB

IF： 5.395

文献链接

标题： Hmox1 is Identified as a Ferroptosis Hub Gene and Associated with the M1 Type Microglia/Macrophage Polarization in Spinal Cord Injury: Bioinformatics and Experimental Validation

样本种属： Rat

杂志： MOLECULAR NEUROBIOLOGY

应用： WB

IF： 5.1

文献链接

标题： Synthesis and biological evaluation of lycoctonine derivatives with cardiotonic and calcium channels inhibitory activities

样本种属： Rat

杂志： BIOORGANIC CHEMISTRY

应用： WB

IF： 5.1

文献链接

标题： Intermittent fasting ameliorates neuronal ferroptosis and cognitive impairment in mice after traumatic brain injury