-
Product Name
beta Actin (Zebrafish Specific) (9E7) Mouse mAb
-
Gene ID
60
-
SwissProt ID
P60709
-
Gene Name
ACTB
-
Alternative Names
ACTIN; ACTB; BETA-ACTIN; beta actin; actin beta
-
Background
beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.
-
Research Field
Cell Biology
-
Product Categories
Loading Control antibody
-
Host
Mouse
-
Reactivity
Zebrafish
-
Application
WB
-
Dilution Ratio
WB: 1/5000-1/10000
-
Molecular Weight
Calculated MW: 42 kDa; Observed MW: 42 kDa
-
Clonality
Monoclonal Antibody
-
Clonality No.
9E7-3F6-6B5
-
Isotype
IgG1
-
Immunogen
Synthetic Peptide of β-actin
-
Purification
Affinity Purified
-
Conjugation
Unconjugated
-
Modification
Unmodified
-
Form
Liquid
-
Buffer System
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
-
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.