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货号： 250133

别名： G3PD; GAPD; HEL-S-162eP

应用： WB,IHC-F,IHC-P,ICC/IF

反应种属： Human,Rat,Mouse,Monkey,Dog,Chicken,Hamster,Rabbit,Pig,Sheep,Insect,Yeast

目录价格：

RMB(含税报价)

数量： - + （库存件）

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产品详情

Product Name

GAPDH (8A3) Mouse mAb
Gene ID

2597
SwissProt ID

P04406
Gene Name

GAPDH
Alternative Names

G3PD; GAPD; HEL-S-162eP
Background

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.
Research Field

Neuroscience
Product Categories

Loading Control antibody
Host

Mouse
Reactivity

Human,Rat,Mouse,Monkey,Dog,Chicken,Hamster,Rabbit,Pig,Sheep,Insect,Yeast
Application

WB,IHC-F,IHC-P,ICC/IF
Dilution Ratio

WB: 1/5000-1/10000 IHC: 1/50-1/200 IF: 1/100-1/200
Molecular Weight

Calculated MW: 36 kDa; Observed MW: 36 kDa
Clonality

Monoclonal Antibody
Clonality No.

8A3-6C3-8D6
Isotype

IgG1
Immunogen

Synthetic Peptide of GAPDH
Purification

Affinity Purified
Conjugation

Unconjugated
Modification

Unmodified
Form

Liquid
Buffer System

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

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验证详情

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应用缩写

WB:蛋白质印迹法IHC-P:石蜡切片样本的免疫组织化学法IHC-F:冰冻切片样本的免疫组织化学法ICC/IF:免疫细胞荧光 IP:免疫沉淀法FC:流式细胞术ChIP:染色质免疫沉淀法ELISA:酶联免疫吸附测定正能生物产品售后保障范围仅限官网产品详情页中“应用”一栏写明的实验应用，请参照上方的缩写含义，仔细对照后选择合适的产品。

引用文献更多完整详情

标题： HSPA8 Activates Wnt/β-Catenin Signaling to Facilitate BRAF V600E Colorectal Cancer Progression by CMA-Mediated CAV1 Degradation

样本种属： Human

杂志： Advanced Science

应用： CoIP,WB

IF： 15.1

文献链接

标题： Tethering ATG16L1 or LC3 induces targeted autophagic degradation of protein aggregates and mitochondria

样本种属： Human

杂志： Autophagy

应用： WB

IF： 13.3

文献链接

标题： Inhibition of Oncogenic Src Ameliorates Silica-Induced Pulmonary Fibrosis via PI3K/AKT Pathway

样本种属： Mouse

杂志： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

应用： IF,WB

IF： 6.21

文献链接

标题： miR-653-3p promotes genomic instability of colorectal cancer cells via targeting SIRT1/TWIST1 signaling pathway

样本种属： Human

杂志： BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

应用： WB

IF： 6.2

文献链接

标题： TREM2 mitigates NLRP3-mediated neuroinflammation through the NF-κB and PI3k/Akt signaling pathways in juvenile rats exposed to ambient particulate matter

样本种属： Rat,Mouse

杂志： ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH

应用： WB

IF： 5.8

文献链接

标题： HucMSC exosomes attenuate partial bladder outlet obstruction-induced renal injury and cell proliferation via the Wnt/β-catenin pathway

样本种属： Mouse,Human

杂志： EUROPEAN JOURNAL OF PHARMACOLOGY

应用： WB

IF： 5.2

文献链接

标题： CircCCDC91 regulates chicken skeletal muscle development by sponging miR-15 family via activating IGF1-PI3K/AKT signaling pathway