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Product Name
beta Actin Rabbit pAb
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Gene ID
60
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SwissProt ID
P60709
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Gene Name
ACTB
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Alternative Names
ACTIN; ACTB; BETA-ACTIN; beta actin; actin beta
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Background
beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.
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Research Field
Cell Biology
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Product Categories
Loading Control antibody
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Host
Rabbit
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Reactivity
Human,Mouse,Rat
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Application
WB,IHC-P,ICC/IF
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Cited
WB (Pig, Chicken,Bird,Helicoverpa zea,Broiler ); IF (Chicken,Pig)
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Dilution Ratio
WB: 1/5000-1/10000 IHC: 1/50-1/200 IF: 1/100-1/200
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Molecular Weight
Calculated MW: 42 kDa; Observed MW: 42 kDa
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Clonality
Polyclonal Antibody
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Clonality No.
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Isotype
IgG
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Immunogen
A synthesized peptide derived from human beta Actin
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Purification
Affinity Chromatography
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
Liquid
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Buffer System
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.