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Product Name
GFP/eGFP Tag (3F11) Mouse mAb
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Gene ID
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SwissProt ID
C8CHS1
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Gene Name
eGFP
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Alternative Names
GFP; Green Fluorescent Protein; enhanced Green Fluorescent Protein.
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Background
Swiss-Prot Acc.C8CHS1.Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage and defines subcellular protein localization.
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Research Field
Tags & Cell Markers
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Product Categories
Tag antibody
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Host
Mouse
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Reactivity
Species-independent
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Application
WB
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Dilution Ratio
WB: 1/1000-1/10000
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Molecular Weight
Calculated MW: 27 kDa; Observed MW: Refer to figures
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Clonality
Monoclonal Antibody
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Clonality No.
3F11-B12-E7-F12
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Isotype
IgG1
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Immunogen
Purified recombinant EYFP.tag full length expressed in E.coli.
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
Liquid
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Buffer System
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.