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Product Name
Flag Tag (10E1) Mouse mAb
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Gene ID
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SwissProt ID
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Gene Name
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Alternative Names
DYKDDDDK Tag; Flag; FLAG
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Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.
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Research Field
Tags & Cell Markers
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Product Categories
Tag antibody
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Host
Mouse
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Reactivity
Species-independent
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Application
WB,IP
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Dilution Ratio
WB: 1/1000-1/10000 IP: 1/500
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Molecular Weight
Refer to figures
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Clonality
Monoclonal Antibody
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Clonality No.
10E1-1D8-5F5
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Isotype
IgG2b
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Immunogen
Monoclonal antibodies are produced by immunizing mouse with synthetic peptide (DYKDDDDK), coupled to KLH.
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
Liquid
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Buffer System
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.