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Product Name
[KO] Phospho-4E BP1 (Thr46) Rabbit mAb
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Gene ID
1978
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SwissProt ID
Q13541
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Gene Name
EIF4EBP1
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Alternative Names
EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
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Background
Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity.
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Research Field
Epigenetics and Nuclear Signaling
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Product Categories
KO/KD validated
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Host
Rabbit
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Reactivity
Human,Mouse,Rat
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Application
WB,IHC-P,IP
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Dilution Ratio
WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20
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Molecular Weight
Calculated MW: 13 kDa; Observed MW: 15-20 kDa
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Clonality
Monoclonal Antibody
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Clonality No.
R03-5C7
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Isotype
IgG
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Immunogen
A synthetic phosphopeptide corresponding to residues surrounding Thr45 of human eIF4EBP1
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Phosphorylated
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Form
Liquid
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Buffer System
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.