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Product Name
[KO] PMS2 Mouse mAb
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Gene ID
5395
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SwissProt ID
P54278
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Gene Name
PMS2
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Alternative Names
DNA mismatch repair gene; DNA mismatch repair protein PMS2; HNPCC4; PMS1 protein homolog 2
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Background
Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected.
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Research Field
Epigenetics and Nuclear Signaling
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Product Categories
KO/KD validated
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Host
Mouse
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Reactivity
Human
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Application
WB,IHC-P,ICC/IF
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Dilution Ratio
WB: 1/500-1/1000 IHC: 1/50-1/100 ICC: 1/100-1/200
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Molecular Weight
Calculated MW: 96 kDa; Observed MW: 150 kDa
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Clonality
Monoclonal Antibody
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Clonality No.
2G5
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Isotype
IgG2b
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Immunogen
Full length human recombinant protein of human PMS2 produced in 293T cell.
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Purification
Affinity Purified
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Conjugation
Unconjugated
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Modification
Unmodified
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Form
Liquid
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Buffer System
Liquid in PBS containing 50% glycerol, 1% BSA and 0.02% sodium azide, pH 7.3.
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Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.